Download Amino Acid Analysis Protocols (Methods in Molecular Biology by Cooper C., Packer N. PDF

By Cooper C., Packer N.

Proteome structures Ltd, North Ryde, Australia. Describes numerous amino acid research recommendations and the way each one method can be utilized to respond to particular biologic questions.

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A. and Swaider, J. M. (1992) Chromatography of 99 amino acids and other ninhydrin-reactive compounds in the Pickering lithium gradient system. J. Chromatog. 594, 165–171. 20. Clarke, A. , Rocklin, R. , and Avdalovic, N. (1999) An integrated amperometry waveform for the direct, sensitive detection of amino acids and amino sugars following anion-exchange chromatography. Anal. Chem. 71, 2774– 2781. 21. Strydom, D. J. (1996) Amino acid analysis using various carbamate reagents for precolumn derivatization, in Techniques in Protein Chemistry VII (Marshak, D.

Hydrate the gel with 500 µL of HPLC-grade water for 15 min at room temperature (see Note 6) and then centrifuge the column for 4 min at about 2000g to equilibrate the column. 2. Wash the column 3 times with 500 µL of water, to ensure full equilibration. 3. Blot the exterior of the column dry to remove all moisture and place it in a new collecting tube. For the macrospin columns, add 50–150 µL of sample to the top, being careful to place the sample in the center of the column. 4. Centrifuge the tube for 4 min at 2000g, and wash the column with at least 200 µL of water (see Note 7).

And Plummer, T. H. (1985) Deglycosylation of asparagine-linked glycans by peptide: N-glycosidase F. Biochemistry 24, 4665– 4671. 12. , Tabak, L. , and Ramasubbu, N. (1991) The influence of flanking sequences on O-glycosylation. Biochem. Biophys. Res. Commun. 180, 1024–1030. 13. Wilson, I. B. , and von Heijne, G. (1991) Amino acid distributions around O-linked glycosylation sites. Biochem. J. 275, 528–534. 14. , Packer, N. , Redmond, J. , Williams, K. , and Gooley, A. A. (1994) Characterization of O-linked glycosylation motifs in the glycopeptide domain of bovine κ-casein.

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