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By Abelson J.N., Simon M.I., Phillips I.M.

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Hesdorffer, J. Ayello, M. Ward, A. Kaubisch, L. Vahdat, C. Balmaceda, T. Garrett, M. Fetell, R. Reiss, A. Bank, and K. Antman,. J. Clin. Oncol. 16(1), 165 (1998). 26 M. Onodera, T. Ariga, N. Kawamura, I. Kobayashi, M. Ohtsu, M. Yamada, A. Tame, H. Furuta, M. Okano, S. Matsumoto, H. Kotani, G. J. McGarrity, R. M. Blaese, and Y. Sakiyama, Blood 91(1), 30 (1998). 38 ANTISENSE RECEPTOR TARGETS 131 brosidase to patients with Gaucher disease; 27 IL-2z8 or p5329 gene transfer for advanced lung cancer patients; and transfer of the herpes simplex virus thymidine kinase gene, followed by ganciclovir administration for treatment of brain tumor, metastases, and melanoma.

2 N N a O H - I % sodium dodecyl sulfate (SDS). MIX with a glass pipette, using it as a rod. 7. Incubate on ice for 5-10 min. 8. 2; mix with the same pipette. 9. 2 ml of chloroform; mix. 10. Incubate on ice for 20 min. 11. Spin at 3000g at 4° for 20 min. 12. Transfer supernatant to a fresh bottle. 53X. Ye, V. M. Rivera, P. Zoltick, F. Cerasoli, M. A. Schnell, G. Gao, J. V. Hughes, M. Gilman, and J. M. Wilson,Science283, 88 (1999). ANTISENSE DNA DELIVERY FOR PROLONGED EFFECTS 43 Plasmid - Adeno-Associated Viral vector GENE PACKAGING INHIBITION OF PROTEIN (5) GENE EXPRESSION COMPETITION (4)GENE INTEGRATION Firm .

S. , Regul. Pept. 59, 193 (1995). 16B. J. Chiasson, J. N. Armstrong, M. L. Hooper, P. R. Murphy, and H. A. Robertson, Cell. Mol. Neurobiol. 14, 507 (1994). 17 B. D. Shivers, I. Killisch, R. Sprengel, H. Sontheimer, M. Krhler, P. R. Schofield, and P. H. Seeburg, Neuron 3, 327 (1989). [2] TARGETED GABAA RECEPTORS 27 m R N A is often used in in vivo antisense studies involving CNS proteins and has been successful in a number of studies targeting different neuronal proteins. The nucleotide sequences of antisense ODNs used to target the 3"2 subunit as well as control ODNs are shown in Table I.

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