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By John N. Abelson, Melvin I. Simon, Willis A. Wood, Scott T. Kellogg

The seriously acclaimed laboratory usual, Methods in Enzymology , is likely one of the such a lot hugely revered guides within the box of biochemistry. considering that 1955, every one quantity has been eagerly awaited, often consulted, and praised via researchers and reviewers alike. The sequence includes a lot fabric nonetheless correct this present day - actually a vital e-book for researchers in all fields of existence sciences.

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Olofsson, J. Wikstrom, and J. W. Wood, J. Agric. Food Chem. 25, 704 (1977). [4] DIOXANE LIGNIN PREPARATIONS 31 the biodegradability of industrial lignins (prepared from [|4C]lignin-labeled lignocelluloses)," comparisons of the biodegradabilities of different species of wood lignins, 3 and biodegradation of lignin within the guts of invertebrates. 45 44D. L. Crawford,S. Floyd,A. L. Pometto III, and R. L. Crawford,Can. J. Microbiol. 23, 434 (1977). 45j. H. A. Butlerand J. C. Buckerfield,Soil Biol.

Food Chem. 25, 704 (1977). [4] DIOXANE LIGNIN PREPARATIONS 31 the biodegradability of industrial lignins (prepared from [|4C]lignin-labeled lignocelluloses)," comparisons of the biodegradabilities of different species of wood lignins, 3 and biodegradation of lignin within the guts of invertebrates. 45 44D. L. Crawford,S. Floyd,A. L. Pometto III, and R. L. Crawford,Can. J. Microbiol. 23, 434 (1977). 45j. H. A. Butlerand J. C. Buckerfield,Soil Biol. Biochem. 11, 507 (1979). [4] P r e p a r a t i o n of Dioxane Lignin Fractions by Acidolysis By BERNARD MONTIES General Principles A convenient method for the preparation of lignin fractions from biomass is provided by the combination of mild solvolytic conditions with a good lignin solvent.

An additional extraction recommended for most [t4C]lignin-labeled lignocellulose involves treatment of the material with proteolytic enzymes. This should always be done if the plant material employed has been labeled by feeding plants L-phenylalanine, since some of the label administered will be incorporated into plant proteins. Labeling of plant proteins usually is not a problem when lignin precursor~, such as cinnamic acid or ferulic acid, are employed. H Protease treatment as outlined by Reid and Seifertt3 or Benner et al.

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