By Mitchelson, Cheng
A good panel of hands-on specialists and builders of CE gear describe in step by step type their top state of the art tools for the detection and research of DNA mutations and differences, starting from unique DNA loci to whole genomes of organisms. this primary quantity of the set, advent to the Capillary Electrophoresis of Nucleic Acids, covers the sensible and theoretical issues at the back of using capillary electrophoresis for the research of small oligonucleotides and transformed nucleotides. in addition to unique directions making sure prepared reproducibility, those protocols provide time-tested recommendation on instrumentation, sign detection, the capillary setting, and the combination of mass spectrometry with CE. numerous chapters are dedicated to the research of small healing oligonucleotides, nucleosides, and ribonucleotides through CE. The better half quantity, useful purposes of Capillary Electrophoresis, addresses thoughts for high-throughput research of DNA fragments utilizing SNP detection, mutation detection, DNA sequencing equipment, and DNA-ligand interactions. entire and updated, the paired volumes of Capillary Electrophoresis of Nucleic Acids provide an authoritative advisor with quick access to speedy, flexible, trustworthy, and robust applied sciences for all these easy and medical investigators examining DNA version this day.
Read or Download Capillary Electrophoresis of Nucleic Acids Volume 1 Introduction to the Capillary Electrophoresis (Methods in Molecular Biology Vol 162) PDF
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Extra resources for Capillary Electrophoresis of Nucleic Acids Volume 1 Introduction to the Capillary Electrophoresis (Methods in Molecular Biology Vol 162)
Mansfield, E. , Wilson, R. , and Fortina, P. (2001) Analysis of short tandem repeat markers by capillary array electrophoresis, in Capillary Electrophoresis of Nucleic Acids, Vol. 2 (Mitchelson, K. R. ), Humana Press, Totowa, NJ, pp. 151–161. 44. Mansfield, E. , Robertson, J. , Isenberg, A. , Frazier, R. , Harris, D. , Barker, D. , Gill, P. , and McCord, B. R. (1998) Analysis of multiplexed short tandem repeat (STR) systems using capillary array electrophoresis. Electrophoresis 19, 101–107. 45.
Ligase Chain Reaction Ligase chain reaction (LCR) is a thermocycler-based assay for known mutations in which oligonucleotide primers fully complementary at loci to particular alleles amplify a defined ligation product, whereas on other alleles, primers do not align fully and are thus refractory to cyclic-ligation. Since the dsDNA ligation products are short (typically ~ 50–100 bp) and can be rapidly separated from unincorporated ligation primers (20–25 bp) the LCR mutation assay is suited to very rapid CE separation techniques.
And Tura, S. (1998) Detection of bcr-abl transcript in chronic myelogenous leukemia patients by reverse-transcription-polymerase chain reaction and capillary electrophoresis. Haematologica 83, 593–601. 70. Borson, N. , Strausbauch, M. , Wettstein, P. , Oda, R. , Johnston, S. , and Landers, J. P. (1998) Direct quantitation of RNA transcripts by competitive single-tube RT-PCR and capillary electrophoresis. Biotechniques 25, 130–137. 71. , and Brandt, B. H. (1998) Direct quantification of polymerase chain reaction fragments using field-amplified sample injection in capillary zone electrophoresis for gene dosage estimation.